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PROCESS FOR EXPRESSION OF RECOMBINANT PROTEINS IN PICHIA PASTORIS USING A FED BATCH MODEL
PROCESS FOR EXPRESSION OF RECOMBINANT PROTEINS IN PICHIA PASTORIS USING A FED BATCH MODEL
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机译：用补料分批培养模型在毕赤酵母中表达重组蛋白的方法
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摘要
The present disclosure relates to a comprehensive model for expression of recombinant peptides by Pichia pastoris. The model uses an easily controllable variable called ‘critical nutrient ratio’ for obtaining a right balance between product synthesis and it's degradation during the fermentation process. The extra cellular concentration of precursor could be increased by about 10 folds and the degradation constants could be reduced by about 10-20 folds for intracellular and extracellular cases respectively by controlling critical nutrient ratio and addition of soya flour hydrolysate and EDTA.
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