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METHOD FOR ASSAYING FATTY ACIDS IN ERYTHROCYTES

机译:评估红细胞中脂肪酸的方法

摘要

The invention relates to a method for dosing erythrocyte fatty acids, comprising the following steps: supply of a volume of a sample of human blood containing erythrocyte fatty acids and constituent components of blood; production of a concentrated solution of the fatty acids; esterification in order to form a solution containing methyl esters derived from said erythrocyte fatty acids; extraction of the methyl esters from the solvent, said extraction step comprising an evaporation step; dosing by means of gas chromatography coupled with a flame ionization detector in order to obtain a measurement of the quantity of said methyl esters; comparison of said measured quantities with pre-determined values of quantities of methyl esters in order to dose said erythrocyte fatty acids.
机译:本发明涉及一种定量红细胞脂肪酸的方法,该方法包括以下步骤:提供一定体积的含有红细胞脂肪酸和血液组成成分的人血样品;生产脂肪酸的浓缩溶液;酯化以形成含有衍生自所述红血球脂肪酸的甲酯的溶液;从溶剂中萃取甲酯,所述萃取步骤包括蒸发步骤;通过气相色谱法和火焰离子化检测器配量,以获得所述甲酯的量的测量值;将所述测得的量与甲基酯的预定量的值进行比较,以定量所述红细胞脂肪酸。

著录项

  • 公开/公告号EP3137911B1

    专利类型

  • 公开/公告日2019-09-11

    原文格式PDF

  • 申请/专利权人 SYNLAB BELGIUM;

    申请/专利号EP20140720962

  • 发明设计人 VERHOEFT ANDRÉ;

    申请日2014-04-29

  • 分类号G01N33/92;

  • 国家 EP

  • 入库时间 2022-08-21 12:31:35

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