METHOD FOR ISOLATION OF CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM LUNG TISSUE

• 1. 新疆地区的结核分枝杆菌临床分离株中存在PhoP基因的天然缺失突变菌株 [P] . 中国专利： CN109810932A . 2019-05-28
• 2. 一株HuNoV GII.4临床分离株序列及其感染性克隆的构建与应用 [P] . 中国专利： CN110144331B . 2021.10.08
• 3. Method for isolating DNA of clinical isolates of Mycobacterium tuberculosis from lung tissue [P] . 俄罗斯专利： RU2016129359A . 2018-01-23

  机译： 从肺组织中分离结核分枝杆菌临床分离株的DNA的方法

• 4. METHOD FOR DETECTING MYCOBACTERIUM OF TUBERCULOSIS FROM RESECTED TISSUE OF LUNG [P] . 俄罗斯专利： RU2009122544A . 2010-12-20

  机译： 从肺切除的组织中检测结核分枝杆菌的方法

• 5. A Diagnostic Kit for simultaneously detection of Clinical Strains of Mycobacterium tuberculosis, Mycobacterium bovis and/or Mycoabcterium bovis BCG and method using the same [P] . 韩国专利： KR101512341B1 . 2015-04-15

  机译： 同时检测结核分枝杆菌，牛分枝杆菌和/或牛分枝杆菌卡介苗临床菌株的诊断试剂盒及其使用方法

• 1. Rapid Detection of Rifampin-resistant Clinical Isolates of Mycobacterium tuberculosis by Reverse Dot Blot Hybridization [J] . GUO Qian, WAN Kang Lin, YU Yan, 生物医学与环境科学（英文版） . 2015,第001期
• 2. Luciferase reporter phage phAE85 for rapid detection of rifampicin resistance in clinical isolates of Mycobacterium tuberculosis [J] . Gomathi Sivaramakrishnan, Balaji Subramanyam, Ponnuraja C, 亚太热带医药杂志（英文版） . 2013,第009期
• 3. Detection of mutation in embB gene of Mycobacterium tuberculosis from clinical isolates of tuberculous patients in China by means of reverse-dot blot hybridization [J] . XUE QIONG WU, YANG LU, JIAN QIN LIANG, 中华微生物学和免疫学杂志（英文版） . 2006,第001期
• 4. DETECTION OF RESISTANCE TO ANTI-TUBERCULOSIS DRUGS IN THE CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS FROM SLOVAKIA THROUGH COMPARISON BETWEEN PHENOTYPIC AND GENETIC METHODS AND EVALUATION OF RESISTANCE LEVELS WITH CLINICAL PARAMETERS [J] . J.MOKRY, I.PORVAZNIK, P.KUSNIR, Journal of physiology and pharmacology: an official journal of the Polish Physiological Society . 2019,第1期
• 5. Assess drug resistance pattern and genetic profile of Mycobacterium tuberculosis clinical isolates by molecular typing methods using direct repeats and IS6110 in pulmonary tuberculosis cases [J] . Deepika Kalo, Surya Kant, Kanchan Srivastava, Lung India . 2017,第2期
• 6. Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method [J] . Basit Abdul, Hussain Mubbashir, Ayaz Sultan, Indian Journal of Animal Research . 2015,第5期
• 7. Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis [C] . Ni Made Mertaniasih, Didik Handijatno, Agnes Dwi Sis Perwitasari, International Seminar on Molecular and Cellular Life Sciences . 2016
• 8. Genomic analyses of Mycobacterium species and clinical Mycobacterium tuberculosis isolates from Utah. [D] . Albert, Heather Ann Hall. 2001
• 9. The MB/BacT Is a Sensitive Method of Isolating Mycobacterium tuberculosis from Clinical Specimens in a Laboratory with a Low Rate of Isolation [O] . William H. Benjamin Jr., Ken B. Waites, Stephen A. Moser, 2000
• 10. Assess drug resistance pattern and genetic profile of Mycobacterium tuberculosis clinical isolates by molecular typing methods using direct repeats and IS6110 in pulmonary tuberculosis cases [O] . Deepika Kalo, Surya Kant, Kanchan Srivastava, 2017

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. First Line Anti-Tuberculosis Drugs Resistance Patterns of iMycobacterium tuberculosis/iIsolates from Newly Diagnosed Cases of Tuberculosis [J] . Yogita Mistry ,Sangita Rajdev ,Summaiya Mullan . 医学微生物学（英文） . 2017,第3期
• 3. MTBDR plus方法快速检测北京地区临床结核分枝杆菌分离株利福平和异烟肼耐药性效果评价 [C] . 菅记涌 ,王嫩寒 ,易俊莉 . 2010年中国防痨协会临床/基础专业学术大会 . 2010
• 4. 结核分枝杆菌临床分离株耐药基因突变位点的检测及诊断抗原的筛选 [A] . 姬祥 . 2018