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A Microfluidic device for analyzing chemotaxis of microalgae and Method for analyzing chemotaxis by using the same

机译:用于分析微藻趋化性的微流体装置和使用其的分析趋化性的方法

摘要

The invention microalgae chemotaxis assay microfluidic devices, and relates to a chemotaxis assay of algae using the same, and more particularly microalgae storage unit on a solid substrate for; Flow channel which is formed a concentration gradient of the coin parameter (chemoattractant), the microalgae movement; Gwanchalbu to observe the microalgae moved; But microalgae is not passed, agarose channel is semi-permeable material which is diffusion-filled coin factor; And the coin storage factor and including a, relates to a flow channel, and the agar microfluidic device with a height of a rose channel to analyze the chemotaxis of microalgae of 15-30μm and a chemotaxis assay method of microalgae using the same. microfluidic device according to the invention without the use of complex dual-layer (Two-layer) lithography (lithography) method, to thereby prepare in a simple single-layer lithography method by a production is not only simple, addition a separate observation presence the microalgae can be quantitatively analyzed at the single cell stage, it is useful to analyze the chemotaxis of microalgae.;
机译:本发明的微藻趋化性测定微流体装置,并且涉及使用藻类的趋化性测定,更具体地涉及在固体基质上用于微藻的储存单元;流道形成了硬币参数(化学吸引剂),微藻运动的浓度梯度; Gwanchalbu观察微藻的移动;但是微藻没有通过,琼脂糖通道是半渗透性物质,是弥散性的硬币因子。并且,包含a的硬币储藏系数涉及流道,具有上升通道的高度的琼脂微流体装置,用于分析15〜30μm的微藻的趋化性以及使用该琼脂微流体装置的微藻的趋化性测定方法。无需使用复杂的双层(Two-layer)光刻法(lithography)的根据本发明的微流体装置,从而通过简单的单层光刻法制备方法不仅生产简单,而且另外存在单独的观察可以在单细胞阶段对微藻进行定量分析,这对于分析微藻的趋化性很有用。

著录项

  • 公开/公告号KR101845528B1

    专利类型

  • 公开/公告日2018-04-04

    原文格式PDF

  • 申请/专利权人 고려대학교 산학협력단;

    申请/专利号KR20160009449

  • 发明设计人 김영환;최홍일;심상준;

    申请日2016-01-26

  • 分类号C12Q1/06;B01L3/00;

  • 国家 KR

  • 入库时间 2022-08-21 12:38:06

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