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Spectral analysis on the light reflection for measuring nanostructure of the myelinated axon

机译:测量髓鞘轴突纳米结构的光反射光谱分析

摘要

The present invention relates to a method for analyzing a nanostructure of a nerve axon forming a medullary sheath using a reflection spectroscopy, and a device used therein. More specifically, the present invention relates to a method for optically analyzing an optical structure of a nerve axon forming a medullary sheath through a reflection spectroscopy, which analyzes a spectrum of an optical signal reflected through an optical element by extracting an optical reflection signal from a focus through a confocal optical system. Therefore, the analysis method according to the present invention can track and observe physiological and pathological states of a nerve axon cell organelle. The method comprises the following steps of: sequentially converting light generated from a white light source into monochrome (S1); entering the monochromatic light in the step S1 into a specimen (S2); and detecting the reflected light generated from the specimen in the step S2 at an optical detector by passing through a scanner and an object lens and passing through a lens for a confocal observation and a pin hole through a beam splitter.
机译:本发明涉及一种使用反射光谱法分析形成髓鞘的神经轴突的纳米结构的方法,以及其中使用的装置。更具体地,本发明涉及一种通过反射光谱对形成髓鞘的神经轴突的光学结构进行光学分析的方法,该方法通过从光学元件中提取光学反射信号来分析通过光学元件反射的光学信号的光谱。通过共焦光学系统聚焦。因此,根据本发明的分析方法可以跟踪和观察神经轴突细胞器的生理和病理状态。该方法包括以下步骤:将由白光源产生的光顺序地转换为单色(S1);在步骤S1中将单色光入射到样本中(S2);在步骤S2中,通过扫描仪和物镜并通过用于共焦观察的透镜和通过分束器的针孔,在光学检测器处检测在步骤S2中从样品产生的反射光。

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