A polyvinyl alcohol/sodium alginate/hydroxyapatite composite fiber membrane, a preparation method and an application thereof. The method for preparing the composite fiber membrane comprises: first preparing hydroxyapatite by using a diammonium hydrogen phosphate and calcium nitrate reaction, and ultrasonically dispersing the hydroxyapatite and sodium alginate into a stable hydroxyapatite suspension; then using the suspension to prepare a sodium alginate solution having a mass fraction of 2% and a polyvinyl alcohol solution having a mass fraction of 18% respectively; finally, mixing the two solutions evenly in proportion and performing electrostatic spinning.
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