Immunochromatographic analysis device for detecting gliadin, immunochromatrographic analysis kit and immunochromatographic analysis method

• 1. 麦醇溶蛋白检测用免疫层析分析装置、免疫层析分析试剂盒以及免疫层析分析方法 [P] . 中国专利： CN108700571A . 2018-10-23
• 2. 免疫色谱试验片及色谱分析方法 [P] . 中国专利： CN100430726C . 2008.11.05
• 3. IMMUNOCHROMATOGRAPHIC ANALYSIS DEVICE FOR DETECTING GLIADIN, IMMUNOCHROMATROGRAPHIC ANALYSIS KIT AND IMMUNOCHROMATOGRAPHIC ANALYSIS METHOD [P] . 世界知识产权组织专利： WO2017146118A1 . 2017-08-31

  机译： 检测胶质蛋白的免疫层析分析装置，免疫层析分析试剂盒和免疫层析分析方法

• 4. Immunochromatographic analyzer for detecting gliadin, immunochromatographic analysis kit and immunochromatographic analysis method [P] . 日本专利： JP6738888B2 . 2020-08-12

  机译： 用于检测麦醇溶蛋白的免疫色谱分析仪，免疫色谱分析试剂盒和免疫色谱分析方法

• 5. DEVICE IMMUNOCHROMATOGRAPHIC ANALYSIS, METHOD OF IMMUNOCHROMATOGRAPHIC ANALYSIS AND KIT FOR IMMUNOCHROMATOGRAPHIC ANALYSIS [P] . 俄罗斯专利： RU2016146092A . 2018-05-28

  机译： 设备免疫层析分析，免疫层析分析方法和用于免疫层析分析的试剂盒

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• 5. Development of a Rapid Diagnostic Kit That Uses an Immunochromatographic Device To Detect Antibodies in Human Sparganosis [J] . Hiroshi Yamasaki, Takeshi Nakamura, Pewpan M. Intapan, Clinical and vaccine immunology: CVI . 2014,第9期
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• 9. Development of a Rapid Diagnostic Kit That Uses an Immunochromatographic Device To Detect Antibodies in Human Sparganosis [O] . Hiroshi Yamasaki, Takeshi Nakamura, Pewpan M. Intapan, 2014
• 10. Development of a Rapid Diagnostic Kit That Uses an Immunochromatographic Device To Detect Antibodies in Human Sparganosis [O] . Hiroshi Yamasaki, Takeshi Nakamura, Pewpan M. Intapan, 2014

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. A Method for Automating Signal Analysis from Therapeutic Devices [J] . Mohammed A. Eslami ,Joshua W. Guag . 智能控制与自动化（英文） . 2014,第2期
• 3. 免疫亲和层析净化高效液相色谱法与酶联免疫分析法试剂盒检测花生及玉米中黄曲霉毒素 [C] . 费得利 乌帕曼 ,王宏 . 2010第三届国际食品安全高峰论坛 . 2010
• 4. 便携式微量免疫层析色谱分析仪的开发 [A] . 郑宇 . 2016