首页> 外国专利> New Trichoderma atroviride G79/11 fungal strain, method for obtaining biopreparation for methane fermentation of organic wastes using this strain and method for conducting methane fermentation of organic wastes using the biopreparation.

New Trichoderma atroviride G79/11 fungal strain, method for obtaining biopreparation for methane fermentation of organic wastes using this strain and method for conducting methane fermentation of organic wastes using the biopreparation.

机译:新的木霉属阿troviride G79 / 11真菌菌株,使用该菌株获得用于有机废物甲烷发酵的生物制备方法,以及使用该生物制剂进行有机废物甲烷发酵的方法。

摘要

a new fungus trichoderma atroviride strain revealed g79 / 11 deposited in the culture collection of the institute of international industrial biotechnology of the agro food industry in warsaw under the number kkp 2056p, selected from sludge of sewage treatment plant to dairy produce at the same time, for the following enzymes lityczne high activity: celulaz,ksylanaz and beta glucosidase activity and the accompanying: lactase, amylase and protease enzymes pektynolitycznych, used to receive biopreparatu to optimise a methane fermentation.in addition, the invention includes receiving biopreparatu to a methane fermentation method, namely that applies strain fungus trichoderma atroviride g79 / 11, which grows less in stationary culture under aerobic conditions at ph 4.8 and 5.1 in temperature's 80.6 during 14 - 20 days. in the medium containing 1.0 l: lactose in volume 17 - 18 g / l,microcrystalline cellulose of 11 to 12 g / l and soy flour in volume 17 - 18 g / l kh2po4 in volume 6 - 7 g / l, (nh) 2so4 of 9 - 10 g / l, and other ingredients, and then infusing into it namnou017conym inoculum production and leads 2 substrate culture on substrate production with awieraju0105cym in 1.0 l: lactose of 4.5 - 5.5 g / l, microcrystalline cellulose of 9.5 - 105 g / l and soy flour of 19.5 - 20.5 g / l kh2po4 in volume 6 - 7 g / l nh4no3 of 2.50 g / l, apply, cacl2 x 2h2o and mgso4 x 7h2o in similar quantities, as in stationary culture, and other ingredients, and livestock is in aerobic conditions at ph 4.5, in t emperaturze 22 -'s 80.6, during 5 - 10 days.method of a methane fermentation also revealed using biopreparatu in which to apply mixture fermentation waste composition: waste of processing fruit - 22 - 28%, sludge from sewage treatment plant of dairy - 22 - 28%, silage maize - 11 - 13% and exp.code ar rat - 35 - 40% and the mixture is advantageously pre hydrolysis.
机译:一株新的木霉属阿特罗韦霉菌菌株g79 / 11保藏在华沙农业食品工业国际工业生物技术研究所的培养物中,编号为kkp 2056p,同时从污水处理厂的污泥到乳制品中选择,对于下列酶lityczne具有高活性:celulaz,ksylanaz和β葡萄糖苷酶活性以及伴随的乳糖酶,淀粉酶和蛋白酶pektynolitycznych,用于接受生物准备以优化甲烷发酵。此外,本发明包括接受甲烷发酵的生物准备。的方法,即使用真菌木霉atroviride g79 / 11,该菌株在有氧条件下于ph值为4.8和5.1,温度为80.6的固定培养中生长14-20天时较少。在含有1.0升的培养基中:乳糖17-18 g / l,微晶纤维素11-12 g / l,大豆粉17-18 g / l kh2po4 6-7 g / l ,(nh)2so4(9-10 g / l)和其他成分,然后注入其中,使用namno u017conym接种物生产,并在1.0 l的awieraj u0105cym的底物生产中引导2底物培养:乳糖4.5-5.5 g每微升9.5-105 g / l的微晶纤维素和19.5-20.5 g / l kh2po4的大豆粉,体积为6-7 g / l 2.50 g / l的nh4no3,应用cacl2 x 2h2o和mgso4 x 7h2o的数量与固定培养和其他成分中的数量相似,并且牲畜处于有氧条件下,ph值为4.5,在tperaturaturze 22-80.6的情况下,持续5-10天。混合发酵废物的组成成分:加工水果的废物-22-28%,乳制品污水处理厂的污泥-22-28%,青贮玉米-11-13%和xp.code ar rat-35-40%,并且混合物有利地是预水解的。

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