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SYSTEM FOR DETECTING PRESENCE OF A PLURALITY OF DIFFERENT TARGET SEQUENCES IN A SAMPLE

机译:样本中多个不同目标序列的存在性检测系统

摘要

A system for detecting presence of a plurality of different target sequences in a sample, wherein the system is configured to carry out plurality of polymerase chain reaction cycles (PCR), on a mixture comprising the sample, for each of the target sequences to result in respective PCR products if the target sequences are present. The system is also configured to carry out high resolution melt and measure capacitance of the mixture during the high resolution melt for detecting presence of melting temperatures based on the measured capacitance thereby enabling detection of presence of different target sequences in the sample. The PCR products, so formed, are subjected to restriction digestion by addition of enzymes for additional identification of PCR products and thereby the target sequence. Figure 3A
机译:一种用于检测样品中多个不同靶序列的存在的系统,其中该系统被配置为对包含靶样品的混合物中的每个靶序列进行多个聚合酶链反应循环(PCR),以产生如果存在靶序列,则各自PCR产物。该系统还被配置为执行高分辨率熔体并在高分辨率熔体期间测量混合物的电容,以基于所测量的电容来检测熔解温度的存在,从而使得能够检测样品中不同靶序列的存在。这样形成的PCR产物通过加入酶进行限制性消化,以进一步鉴定PCR产物,从而鉴定靶序列。图3A

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