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ANDROGENETIC HAPLOID EMBRYONIC STEM CELL, AND PREPARATION METHOD AND USE THEREOF

机译:雄激素的半胚性胚胎干细胞及其制备方法和用途

摘要

The present invention relates to an AG-haESCs in which H19 DMR and IG-DMR are knocked out, a method for preparing the AG-haESCs, and use of the AG-haESCs in constructing a genetically modified semi-cloned animal and a library of a genetically modified semi-cloned animal. The AG-haESCs is capable of obtaining characteristics resembling a round spermatid, and upon injection into an oocyte, a viable SC mouse is stably obtained. The present invention is capable of being effectively used in multi-gene genetic manipulation, advancing the acquisition of animals with multiple genetic modifications.
机译:本发明涉及一种敲除H19 DMR和IG-DMR的AG-haESCs,该AG-haESCs的制备方法以及该AG-haESCs在构建转基因半克隆动物中的用途和该文库。转基因的半克隆动物。 AG-haESCs能够获得类似于圆形精子的特征,并且在注射到卵母细胞中后,稳定地获得了存活的SC小鼠。本发明能够有效地用于多基因遗传操作中,从而促进了具有多种遗传修饰的动物的获取。

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