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Artificial cell membrane comprising a supported lipid bilayer bound to a probe with tunable mobility and method for analyzing interactions between molecules using the same
Artificial cell membrane comprising a supported lipid bilayer bound to a probe with tunable mobility and method for analyzing interactions between molecules using the same
Disclosed herein are an artificial cell membrane including a supported lipid bilayer (SLB) including a substrate and mobility-decreased metal particles bonded onto the substrate; an analysis device or kit including the artificial cell membrane and examining the interactions between molecules, in which one molecule is bonded to the surface of a mobility-decreased metal particle bonded to the artificial cell membrane and the other molecule is bonded to the surface of a mobility-increased metal particle bonded to a lipid at a low valency; a method of examining the interactions between molecules using the analysis device; a kit for quantitative or qualitative analysis of a target material including the artificial cell membrane by plasmonic scattering measurements; and a multiple analysis kit capable of detecting a plurality of target materials using a plurality of metal particles having different plasmonic scattering wavelengths and/or having mobility on a supported lipid bilayer. According to the artificial cell membrane including a supported lipid bilayer containing metal particles attached thereto, the fluidity of the metal particles on the lipid can be controlled by adjusting the number of ligands bonded to the metal particles. Therefore, target molecules for analyzing the interactions therebetween on two types of metal particles having different fluidity are introduced onto the artificial cell membrane, thereby monitoring the movements of the metal particles through plasmonic scattering so as to analyze the interactions between the target molecules. In this case, multiple analysis of simultaneously detecting and quantifying a plurality of target materials using the artificial cell membrane of the present invention, plasmonic scattering wavelengths, and a plurality of particles having different fluidity can be performed.
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