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Artificial cell membrane comprising a supported lipid bilayer bound to a probe with tunable mobility and method for analyzing interactions between molecules using the same
Artificial cell membrane comprising a supported lipid bilayer bound to a probe with tunable mobility and method for analyzing interactions between molecules using the same
The present invention provides an artificial cell membrane comprising a base material and a supported lipid bilayer (SLB) in which metal particles having reduced mobility are bound on the base material; In the analysis apparatus or kit for confirming the interaction between the contained molecules, one molecule is bound to the surface of the metal particle having reduced mobility bound to the artificial cell membrane, and the other molecule has a low valency. An analysis device characterized in that it is bound to the surface of a metal particle having a high mobility and bound to a lipid; a method for confirming an interaction between molecules using the analysis device; and the artificial cell membrane by plasmon scattering measurement. A kit for quantitative or qualitative analysis of a target substance comprising: a plurality of target substances utilizing a plurality of metal particles having different plasmon scattering wavelengths and / or mobility on a supported lipid bilayer It relates multiplex analysis kits that can be detected. The artificial cell membrane including the supported lipid bilayer to which the metal particles of the present invention are bound can control the mobility of the metal particles on the lipid by adjusting the number of ligands bound on the metal particles. Therefore, by introducing a target molecule to be analyzed for interaction onto two metal particles having different mobility and introducing the target molecule onto the artificial cell membrane, the movement of the metal particle is monitored by plasmon scattering, and the target molecule The interaction between them can be analyzed. At this time, the artificial cell membrane of the present invention and a plurality of particles having different mobility on the plasmon scattering wavelength or lipid can be used to perform multiplex analysis for simultaneously detecting and quantifying a plurality of target substances.
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