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Method for determining spatially resolved height information of a sample using a wide field microscope and a wide field microscope

机译:使用广角显微镜和广角显微镜确定样品的空间分辨高度信息的方法

摘要

The present invention relates to a wide field microscope and a method for determining spatially resolved height information of a sample (14) using a wide field microscope. In the wide-field microscope, an irradiation source (1, 52, 53) arranged in the irradiation beam path and a wide-field image in the observation beam path of the sample (14) irradiated in the sample surface (P) are displayed. A detector (17, 33) for capturing, a modulator for color modulation in a direction perpendicular to the sample plane (P) of the illumination beam path or the observation beam path, and each of the wide-field images And an evaluation unit for calculating chromatic confocal height information within the pixel. The method comprises using any broadband illumination source (1) to illuminate the sample (14) in an illumination beam path, color modulating the illumination beam path or detection beam path, and the detection beam path. Capturing at least one wide-field image from sample light having a chromatic confocal component reflected or emitted from the sample in the image, and from the wide-field image, the chromatic confocal component of the detection beam path and the color modulation And determining various height information of the sample for each pixel.
机译:本发明涉及一种广角显微镜和一种使用广角显微镜确定样品(14)的空间分辨高度信息的方法。在宽视野显微镜中,显示了配置在照射光路中的照射源(1、52、53)和照射在试样表面(P)上的试样(14)的观察光路中的广角图像。 。用于捕获的检测器(17、33),用于在与照明光束路径或观察光束路径的样本平面(P)垂直的方向上进行颜色调制的调制器,以及每个广域图像以及用于计算像素内的彩色共焦高度信息。该方法包括使用任何宽带照明源(1)在照明光束路径中照明样品(14),对照明光束路径或检测光束路径以及检测光束路径进行颜色调制。从具有从图像中的样品反射或发射的色共焦成分的样品光以及从宽视场图像,检测光束路径的色共焦成分和色彩调制中捕获至少一个广域图像,并确定各种每个像素的样本高度信息。

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