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Baname shrimp osmotic regulation related functional gene EST-SSR marker, its specific primer and detection method

机译:百名虾渗透调节相关功能基因EST-SSR标记及其特异性引物和检测方法

摘要

The present invention discloses a phantom shrimp osmotic regulation related functional gene EST-SSR marker, a specific primer thereof, and a detection method. The detection method is performed after PCR amplification is performed on a sequence at a microsatellite site by using a specific primer of a vanilla shrimp osmotic pressure regulation-related functional gene EST-SSR marker, using the genome DNA of the shrimp shrimp as a template. The PCR amplification product is typed using a sequenator. The specific primer and detection method for the banana-shrimp osmotic regulation-related functional gene EST-SSR marker used in the present invention can be applied to the genetic structure analysis and molecular marker-assisted breeding of the lobster shrimp species, and particularly, the salt resistance of the lobster shrimp is excellent. It has important application value in the selective breeding of various varieties. [Selection] Figure 1
机译:本发明公开了一种幻影虾渗透调节相关功能基因EST-SSR标记及其特异性引物和检测方法。检测方法是在虾的基因组DNA为模板的情况下,通过使用香草虾渗透压调节相关功能基因EST-SSR标记的特异性引物对微卫星部位的序列进行PCR扩增后进行的。使用测序仪对PCR扩增产物进行分型。本发明中使用的香蕉虾渗透调节相关功能基因EST-SSR标记的特异引物和检测方法,可用于龙虾虾种的遗传结构分析和分子标记辅助育种。龙虾虾的耐盐性极好。它在各种品种的选育中具有重要的应用价值。 [选择]图1

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