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METHODS FOR DISTINGUISHING STRAINS OF FUSOBACTERIUM NUCLEATUM

机译:区分核镰刀菌菌株的方法

摘要

PROBLEM TO BE SOLVED: To provide methods for distinguishing Fusobacterium nucleatum in samples at strain levels.;SOLUTION: The invention provides a method, for at least one region selected from the following groups, comprising detecting the presence or absence of CRISPR-Cas sequence including the region and the length of the sequence. In DNA derived from a sample: a CRISPR region presents at the 3' side of specific Cas genes present in a specific sequence of Fusobacterium nucleatum subsp. animalis genome or a corresponding region; a CRISPR region present at the 3' side of specific Cas genes present in a specific sequence of Fusobacterium nucleatum subsp. polymorphum genome or a corresponding region; a CRISPR region present at the 3' side of specific Cas genes present in a specific sequence of Fusobacterium nucleatum subsp. vincentii genome or a corresponding region; and a CRISPR region present at the 3' side of specific Cas genes present in another specific sequence of Fusobacterium nucleatum subsp. polymorphum genome or a corresponding region.;SELECTED DRAWING: None;COPYRIGHT: (C)2018,JPO&INPIT
机译:解决的问题:提供一种在菌株水平上区分样品中的核梭状芽胞杆菌的方法。解决方案:本发明提供一种用于选自以下组中的至少一个区域的方法,该方法包括检测是否存在CRISPR-Cas序列,包括序列的区域和长度。在源自样品的DNA中:CRISPR区域出现在特定的Cas基因的3'侧,该基因存在于Fusobacterium nucleatum亚种的特定序列中。动物基因组或相应区域;一个存在于特定的Cas基因3'侧的CRISPR区域,该基因存在于Fusobacterium nucleatum亚种的特定序列中。多形体基因组或相应区域;一个存在于特定的Cas基因3'侧的CRISPR区域,该基因存在于Fusobacterium nucleatum亚种的特定序列中。 Vincentii基因组或相应区域; CRISPR区存在于特定Cas基因的3'侧,该Cas基因存在于Fusobacterium nucleatum subsp的另一个特定序列中。多态性基因组或相应区域。;选图:无;版权:(C)2018,JPO&INPIT

著录项

  • 公开/公告号JP2018078823A

    专利类型

  • 公开/公告日2018-05-24

    原文格式PDF

  • 申请/专利权人 KYODO MILK INDUSTRY CO LTD;

    申请/专利号JP20160222636

  • 发明设计人 MATSUMOTO MITSUHARU;SHIMOMURA ARIYOSHI;

    申请日2016-11-15

  • 分类号C12N15/09;C12Q1/68;

  • 国家 JP

  • 入库时间 2022-08-21 13:13:20

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