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HUMAN SEBOCYTE PRECURSOR CELLS, HUMAN SEBOCYTES AND IN VITRO METHODS FOR OBTAINING THE SAME FROM HUMAN INDUCED PLURIPOTENT STEM CELLS (HIPSC)
HUMAN SEBOCYTE PRECURSOR CELLS, HUMAN SEBOCYTES AND IN VITRO METHODS FOR OBTAINING THE SAME FROM HUMAN INDUCED PLURIPOTENT STEM CELLS (HIPSC)
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机译:从人诱导的多能干细胞(HIPSC)中获得相同抗体的人类核细胞前体细胞,人类核细胞和体外方法
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摘要
One aspect of the invention relates to an in vitro method for obtaining hiPSC-derived sebocyte precursor cells comprising the steps of:a) culturing, under feeder layer-free conditions, human induced pluripotent stem cells (hiPSC) in a sebocyte basal medium complemented with a combination of (i) retinoic acid (RA), (ii) bone morphogenetic protein 4 (BMP4) and (iii) epidermal growth factor (EGF), during a period of time ranging from about 5 days to about 15 days, so as to generate multipotent skin precursor cells,b) culturing, under feeder layer-free conditions, the multipotent skin precursor cells obtained at step a) in a sebocyte differentiation basal medium, during a period of time ranging from about 5 days to about 15 days, so as to generate hiPSC-derived sebocyte precursor cells, andc) collecting the hiPSC-derived sebocyte precursor cells obtained at step b).
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