首页> 外国专利> Shewanella oneidensis for synthesizing formic acid and the method of synthesizing formic acid by using same

Shewanella oneidensis for synthesizing formic acid and the method of synthesizing formic acid by using same

机译：沙瓦氏菌合成甲酸的方法及利用该方法合成甲酸的方法

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摘要

The present invention relates to Shewanella oneidensis, a microorganism, for synthesizing formic acid; and a method for synthesizing formic acid by using the same. In the present invention, Shewanella oneidensis was first used for synthesizing formic acid from CO_2 and electrons generated from an electrode; CO_2 reduction catalyzed by Shewanella oneidensis aerobically grown in an LB medium can generate 14.1 mM formic acid after a 24 hour reaction; and particularly, CO_2 reduction catalyzed by Shewanella oneidensis grown in a medium containing fumarate and nitrate under an anaerobic condition can generate formic acid up to 136.84 mM after a 72 hour reaction. Additionally, the average reaction rate for formic acid synthesis in a 72 hour reaction catalyzed by Shewanella oneidensis grown anaerobically in an optimal medium was 3.8 mMh^(-1)g^(-1) wet cell. This value is 9.6 times greater than that obtained from the reaction catalyzed by Methylobacterium extorquens AM1 in a previous study, and thus Shewanella oneidensis can be expected to be beneficially used as a microorganism capable of synthesizing formic acid from CO_2.

机译：本发明涉及一种用于合成甲酸的微生物希瓦氏菌。以及使用其合成甲酸的方法。在本发明中，首先使用沙瓦氏菌（Shewanella oneidensis）从CO_2和电极产生的电子合成甲酸。在LB培养基中有氧生长的拟人希瓦氏菌（Shewanella oneidensis）催化的CO_2还原在24小时反应后可产生14.1 mM甲酸。尤其是，在厌氧条件下，在含富马酸盐和硝酸盐的培养基中生长的沙瓦氏假单胞菌催化的CO_2还原在72小时反应后可生成高达136.84 mM的甲酸。此外，在最佳培养基中厌氧生长的拟人希瓦氏菌在72小时反应中合成甲酸的平均反应速率为3.8 mMh ^（-1）g ^（-1）。该值比在以前的研究中由甲基芽孢杆菌AM1催化的反应获得的值大9.6倍，因此可以预期沙希氏菌被有利地用作能够由CO_2合成甲酸的微生物。

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公开/公告号KR20170121019A

专利类型
公开/公告日2017-11-01

原文格式PDF
申请/专利权人 KWANGWOON UNIVERSITY INDUSTRY-ACADEMIC COLLABORATION FOUNDATION;
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申请/专利号KR20160049724
发明设计人 KIM YONG HWAN;JANG JEONG HO;CHOI EUN GYU;
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申请日2016-04-24
分类号C12N1/20;C12N1/38;C12P7/40;C12R1/01;
国家 KR
入库时间 2022-08-21 13:26:23

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