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Differentiation method of adipose-derived mesenchymal stem cells into neural stem cells neural cells and GABAergic neural cells
Differentiation method of adipose-derived mesenchymal stem cells into neural stem cells neural cells and GABAergic neural cells
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机译：脂肪间充质干细胞向神经干细胞，GABA能神经细胞的分化方法
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摘要
The present invention relates to a method for differentiating adipocyte-derived mesenchymal stem cells into neural stem cells, neurons, and neural neural cells. Specifically, adipocyte-derived mesenchymal stem cells are cultured in a cell culture medium with SB431543, Noggin, (Step 1: pre-treatment step) and then incubated in medium supplemented with B27, N2, and ascorbic acid for 5 days (step 2: nerve induction step), and bFGF And a medium supplemented with purmorphamine and BDNF (Brain-derived neurotrophic factor) to the cell culture of step 3, and further cultured in a medium supplemented with EGF for 5 to 7 days (step 3: proliferation step) The cells were cultured in the same manner as described in Example 1, except that the cells were cultured in a medium supplemented with purmorphamine, BDNF, dbcAMP, and BDNF. The present invention can be used to confirm the molecular mechanisms involved in the development of human neurons or GABA secretory intervening neurons. In addition, the present invention can be used to identify the molecular mechanisms involved in the development of human neurons or GABA secretory intervening neurons, Neurons derived from mesenchymal stem cells or induced GABA secretion intervening neurons may be useful for neurological disease screening and drug screening.;
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