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How to perform site-specific mutations in whole plants through transient gene expression
How to perform site-specific mutations in whole plants through transient gene expression
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机译：如何通过瞬时基因表达在整株植物中进行位点特异性突变
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摘要
The present invention relates to a method for producing a plant, comprising the steps of: obtaining, as a transient expression target, a complete plant that transiently expresses a nuclease different from a target gene fragment in a plant; Under the action of a nuclease, cutting the target fragment; And performing a site-specific transformation of the target fragment through DNA repair of the plant. The present invention also provides a method for performing a site-specific transformation on a target gene fragment in a whole plant through transient expression of the gene. The present invention also provides a method for producing a non-transgenic mutant plant by the above-mentioned method, whereby a non-transgenic mutant plant is provided.
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