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Means and method for expression of authentic human epidermal growth factor and/or basic fibrolast growth factor in cytoplasm and/or culture medium of Escherichia coli
Means and method for expression of authentic human epidermal growth factor and/or basic fibrolast growth factor in cytoplasm and/or culture medium of Escherichia coli
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机译：真人表皮生长因子和/或碱性成纤维细胞生长因子在大肠杆菌的细胞质和/或培养基中表达的方法和方法
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摘要
The present invention relates to an engineered biological system, and in particular an Escherichia coli host. The host is incorporated with a DNA construct for production of at least a first polypeptide. The first polypeptide may be an authentic basic fibroblast growth factor (bFGF). The DNA construct has an insert including, for example, in the sequence of, an expression cassette, an intein sequence and DNA coding for the first polypeptide. The DNA construct is configured to effect the host to secrete the basic fibroblast growth factor (bFGF) in the cytoplasm of the host and/or to excrete to cell medium in which the host is cultured.
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