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Means and methods for the expression of authentic human epidermal growth factor and / or basic fibroblast growth factor in the cytoplasm and / or medium of E. coli
Means and methods for the expression of authentic human epidermal growth factor and / or basic fibroblast growth factor in the cytoplasm and / or medium of E. coli
The present invention relates to an engineered biological system, and in particular an Escherichia coli host. The host is incorporated with a DNA construct for production of at least a first polypeptide. The first polypeptide may be an authentic basic fibroblast growth factor (bFGF). The DNA construct has an insert including, for example, in the sequence of, an expression cassette, an intein sequence and DNA coding for the first polypeptide. The DNA construct is configured to effect the host to secrete the basic fibroblast growth factor (bFGF) in the cytoplasm of the host and/or to excrete to cell medium in which the host is cultured.
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