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PROBES AND PRIMERS FOR THE RAPID AND RELIABLE QUANTIFICATION OF THE GENES SPECIFIC TO PROSTATE CANCER, USING SUITABLE REFERENCE GENES

机译:使用合适的参考基因快速可靠地量化前列腺癌特异基因的问题和原则

摘要

The present invention relates to new sets of primers and probes and to the use thereof for detecting prostate-specific genes, i.e. the PCA3, TMPRSS2- ERG and AMACR genes, and reference genes selected from the RPL5, HPRT1 and HNRNPA1 genes for normalising the target gene expression analysis data from patient samples, whether these are tissue samples or biological fluids. The sets of primers and probes of the target genes and reference genes are more sensitive than those cited in the literature, and will be used in real-time PCR (qPCR) in simplex or multiplex format for a reliable, rapid and less costly quantification.
机译:本发明涉及新的引物和探针组,及其在检测前列腺特异性基因即PCA3,TMPRSS2-ERG和AMACR基因以及选自RPL5,HPRT1和HNRNPA1基因的参考基因中用于标准化靶标的用途。来自患者样本的基因表达分析数据,无论这些样本是组织样本还是生物体液。靶基因和参考基因的引物和探针组比文献中引用的那些更为敏感,将以单纯或多重形式用于实时PCR(qPCR)中,以进行可靠,快速且成本更低的定量。

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