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Kit for detecting Salmonella species by assaying outer membrane porin F (ompF)

机译：通过检测外膜孔蛋白F（ompF）来检测沙门氏菌物种的试剂盒

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The invention relates to a method of detecting the presence of Salmonella in a sample using novel oligonucleotide sequences. Also presented is a kit for putting the method into practice and novel nucleic acid sequences for ompF. The ompF gene was found to be 100% inclusive for Salmonella species and 100% exclusive for non-Salmonella species for the strains tested thus making it an excellent marker for identification of both the species of Salmonella: S. enterica and S. bongori. Two hundred and eighteen isolates belonging to Salmonella enterica (subspecies I-VI) and Salmonella bongori were examined using novel primers designed to detect the ompF gene. The target was present in all the 218 Salmonella isolates including all the subspecies of Salm. enterica and Salm. bongori. The ompF gene was absent in 180 non-Salmonella strains tested. 展开▼

机译：本发明涉及一种使用新的寡核苷酸序列检测样品中沙门氏菌（I）沙门氏菌（Salmonella）的存在的方法。还介绍了用于将该方法付诸实践的试剂盒和ompF的新核酸序列。发现ompF基因对于沙门氏菌属（I）沙门氏菌而言100％包括在内，对于非沙门氏菌（Salmonella）属植物而言100％除外，因此使其成为鉴定这两种细菌的极佳标记沙门氏菌（S. enterica 和 S）的种类。奉爱。使用旨在检测ompF基因的新型引物检查了218株沙门氏菌（亚种I-VI）和 Bongori沙门氏菌的分离株。目标存在于所有218个沙门氏菌分离株中，包括 Salm的所有亚种。 enterica 和 Salm。奉爱。在180个非沙门氏菌菌株中没有ompF基因。 展开▼

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公开/公告号US9410212B1

专利类型

公开/公告日2016-08-09

原文格式PDF

申请/专利权人 APARNA TATAVARTHY;ANDREW C. CANNONS;LOREE C. HELLER;
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申请/专利号US201414535376

发明设计人 APARNA TATAVARTHY;ANDREW C. CANNONS;LOREE C. HELLER;
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申请日2014-11-07

分类号C07H21/04;C12Q1/68;

国家 US

入库时间 2022-08-21 14:29:01

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1. Evolutionary Implication of Outer Membrane Lipoprotein-Encoding Genes ompL1, lipL32 and lipL41 of Pathogenic Leptospira Species [J] . K. Vedhagiri, K. Natarajaseenivasan, P. Chellapandi, 基因组蛋白质组与生物信息学报（英文版） . 2009,第003期

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