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Method of construction of recombinant organisms using multiple genes co-integration

机译:利用多基因共整合构建重组生物的方法

摘要

Provided is a method of simultaneous co-integration of multiple nucleic acid sequences into a microbial organism comprising culturing a microbial organism to be transformed until a growth phase is reached in which a random integration is facilitated; and transforming the microbial organism by mixing the cultured microbial organism with a sample to be co-integrated into the microbial organism with, wherein the sample comprises more than one nucleic acid sequence, and the products generated from the method.
机译:提供了一种将多个核酸序列同时共整合到微生物中的方法,该方法包括培养待转化的微生物直至达到促进随机整合的生长阶段。通过将培养的微生物与要共整合到其中的样品混合来转化微生物,其中样品包含一个以上的核酸序列,以及从该方法产生的产物。

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