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Surface plasmon excitation enhanced fluorescence spectrometer and surface plasmon excitation enhanced fluorescence spectrometer

机译:表面等离子体激元激发增强荧光光谱仪和表面等离子体激元激发增强荧光光谱仪

摘要

[Technical Problem] It is an object to provide a surface plasmon-field enhanced fluorescence spectroscopic measurement method and a surface plasmon-field enhanced fluorescence spectroscopic measurement apparatus that are capable of measuring a fluorescence signal in a precise manner regardless of a type of a light detection means by adjusting a dynamic range of an SPFS apparatus even in the case in which a concentration of an analyte is high. [Solution of Problem] For a surface plasmon-field enhanced fluorescence stereoscopic measurement method wherein an analyte that has been labeled with a fluorescence substance is excited by surface plasmon light that has been generated by applying an excitation light to a metallic thin film and the generated fluorescence is received by a light detection means to thereby detect the analyte, a dynamic range is expanded by adjusting a light amount of the fluorescence that is received by the light detection means.
机译:[技术问题]本发明的目的是提供一种表面等离激元场增强荧光光谱测量方法和表面等离激元场增强荧光光谱测量设备,其能够与光的类型无关地精确地测量荧光信号。即使在分析物浓度高的情况下,通过调节SPFS装置的动态范围来检测检测装置。 [问题的解决方案]对于表面等离激元场增强的荧光立体测量方法,其中用荧光物质标记的分析物被通过向金属薄膜施加激发光而产生的表面等离激元光激发,并且所产生的通过光检测装置接收荧光从而检测分析物,通过调节由光检测装置接收的荧光的光量来扩大动态范围。

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