首页> 外国专利> CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL FOR CONTINUOUSLY PRODUCING CULTURE MEDIUM WHICH CAN SELECT MORE SUITABLE CELL VARIANT

CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL FOR CONTINUOUSLY PRODUCING CULTURE MEDIUM WHICH CAN SELECT MORE SUITABLE CELL VARIANT

机译:带有移动容器的连续培养装置,用于连续生产可以选择更多合适细胞变种的培养培养基

摘要

PROBLEM TO BE SOLVED: To provide a method for continuously culturing plants, animals, or stem cells.SOLUTION: A method for culturing cells which are in no suspension condition comprises the step of a) providing a system of two or more clamps, each capable of taking open and closed positions, the clamps being positioned so as to be able to divide a tube into: 1) an upstream region containing an unused culture medium and an unused surface capable of culturing cells, 2) a downstream region containing a spent culture medium; and 3) a growth chamber region disposed between the upstream and downstream regions for growing the cells on the surface where the culture medium and the living cells can be cultured in the tube (the surface which is an interior of a tube, or continuous fiber in a tube); the step of b) closing the two or more clamps on the tube, and introducing the living cells into the growth chamber region; the step of c) cyclically closing and opening selected two or more clamps to redefine the growth chamber region so as to provide a surface for an additional culture of cells; and the step of d) repeating the step c.
机译:解决的问题:提供一种连续培养植物,动物或干细胞的方法。解决方案:一种用于培养没有悬浮状态的细胞的方法,包括以下步骤:a)提供两个或多个夹具的系统,每个夹具在处于打开和关闭位置的过程中,将夹具定位成能够将管子分成:1)包含未使用的培养基的上游区域和能够培养细胞的未使用的表面,2)包含废培养液的下游区域中; 3)在上游区域和下游区域之间设置的生长室区域,用于在可在管中培养培养基和活细胞的表面(管内部的表面或连续的纤维)中生长细胞。一个管子); b)关闭管子上的两个或多个夹子,并将活细胞引入生长室区域的步骤; c)步骤周期性地关闭和打开选定的两个或多个夹具以重新定义生长室区域,从而为细胞的额外培养提供表面; d)重复步骤c。

著录项

  • 公开/公告号JP2015213515A

    专利类型

  • 公开/公告日2015-12-03

    原文格式PDF

  • 申请/专利权人 DE CRECY EUDES;

    申请/专利号JP20150147247

  • 发明设计人 DE CRECY EUDES;

    申请日2015-07-24

  • 分类号C12N1;C12N5/074;C12N5/0789;C12N5/0775;C12N5/0735;C12N5/04;C12N1/16;C12M3;

  • 国家 JP

  • 入库时间 2022-08-21 14:45:01

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