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CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL FOR CONTINUOUSLY PRODUCING CULTURE MEDIUM WHICH CAN SELECT MORE SUITABLE CELL VARIANT
CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL FOR CONTINUOUSLY PRODUCING CULTURE MEDIUM WHICH CAN SELECT MORE SUITABLE CELL VARIANT
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机译:带有移动容器的连续培养装置,用于连续生产可以选择更多合适细胞变种的培养培养基
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摘要
PROBLEM TO BE SOLVED: To provide a method for continuously culturing plants, animals, or stem cells.SOLUTION: A method for culturing cells which are in no suspension condition comprises the step of a) providing a system of two or more clamps, each capable of taking open and closed positions, the clamps being positioned so as to be able to divide a tube into: 1) an upstream region containing an unused culture medium and an unused surface capable of culturing cells, 2) a downstream region containing a spent culture medium; and 3) a growth chamber region disposed between the upstream and downstream regions for growing the cells on the surface where the culture medium and the living cells can be cultured in the tube (the surface which is an interior of a tube, or continuous fiber in a tube); the step of b) closing the two or more clamps on the tube, and introducing the living cells into the growth chamber region; the step of c) cyclically closing and opening selected two or more clamps to redefine the growth chamber region so as to provide a surface for an additional culture of cells; and the step of d) repeating the step c.
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