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METHOD, TEST-SYSTEM AND PRIMERS FOR DETERMINATION OF HAPLOGROUPS OF HUMAN Y-CHROMOSOME
METHOD, TEST-SYSTEM AND PRIMERS FOR DETERMINATION OF HAPLOGROUPS OF HUMAN Y-CHROMOSOME
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机译：测定人Y染色体染色体组的方法，试验系统和试剂
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摘要
FIELD: medicine.SUBSTANCE: inventions relate to the field of DNA-genealogy and deal with a method of determining haplogroups of human Y-chromosomes, a test-system and oligonucleotide primers. The characterised method is realised in two stages. At the first stage genetic typing is carried out by basic haplogroups R,N,J,I,Q,C,E,D,G,O of an Y-chromosome tree by multiplex PCR with the application of specific oligonucleotide primers of the first and second sets of the test-system. At the second stage, if mutation is detected, additional multiplex PCR for typing by subhaplogroups is carried out. If at the first stage mutation is not detected, multiplex PCR for typing by rare haplogroups A,C3,F,H,K,L,O3 and T is carried out. The area of annealing primers outside mutation zones within the limits of species specificity corresponds to sequences from the first set of the test-system. The area of annealing of the oligonucleotide primers, directly adjoining the mutation zone within the limits of species specificity corresponds to sequences from the second set of the test-system.EFFECT: inventions can be applied for the determination of haplogroups of the human Y-chromosome.3 cl, 2 dwg, 7 tbl, 2 ex
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机译：技术领域本发明涉及DNA遗传学领域，并涉及确定人Y染色体单倍型的方法，测试系统和寡核苷酸引物。该特征化方法分两个阶段实现。在第一阶段，通过多重PCR结合Y染色体树的基本单倍基团R，N，J，I，Q，C，E，D，G，O进行遗传分型，并应用第一个的特定寡核苷酸引物第二套测试系统。在第二阶段，如果检测到突变，则进行额外的多重PCR，以按亚单倍型进行分型。如果在第一阶段未检测到突变，则进行用于稀有单倍群A，C3，F，H，K，L，O3和T的分型PCR。在物种特异性范围内，突变区外的退火引物区域对应于来自第一套测试系统的序列。寡核苷酸引物的退火区域，在物种特异性范围内直接邻接突变区，对应于第二套测试系统中的序列。效果：本发明可用于测定人Y染色体的单倍型.3 cl，2 dwg，7 tbl，2 ex
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