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METHOD OF DETECTING MUTATIONS IN GENE MYO7A, ACCOMPANIED BY DEVELOPMENT OF NON-SYNDROMIC AUTOSOMAL RECESSIVE DEAFNESS AND USHER SYNDROME
METHOD OF DETECTING MUTATIONS IN GENE MYO7A, ACCOMPANIED BY DEVELOPMENT OF NON-SYNDROMIC AUTOSOMAL RECESSIVE DEAFNESS AND USHER SYNDROME
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机译：检测非基因型自体持续性耳聋和综合征的伴发基因Myo7A中突变的方法
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摘要
FIELD: biotechnology.SUBSTANCE: method comprises isolation of DNA from lymphocytes in peripheral blood by the method of phenol-chloroform extraction, carrying out of PCR, amplification of 18 parts of gene MYO7A, detection in the denaturing acrylamide gel and sequencing. PCR is carried out using specially selected sequences of oligonucleotides flanking regions of 18 exons of the gene MYO7A with possible content of different mutations.EFFECT: invention enables to simplify the method and to improve the accuracy of determining mutations of the gene MYO7A, to reduce the time of the study.3 dwg, 1 ex
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机译：领域：生物技术。方法：该方法包括通过酚-氯仿提取方法从外周血淋巴细胞中分离DNA，进行PCR，扩增18个MYO7A基因，在变性丙烯酰胺凝胶中检测并测序。使用MYO7A基因18个外显子侧翼区域的特殊选择的寡核苷酸序列进行PCR，该序列可能包含不同的突变含量。学习时间。3 dwg，1 ex
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