首页> 外国专利> METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli pREP4, pCFP10-DBD STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION

METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli pREP4, pCFP10-DBD STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION

机译：基于CFP10-DBD杂蛋白和右旋糖的免疫原性组成的获得方法pCFP10-DBD重组质粒;大肠杆菌[pREP4，pCFP10-DBD] STRAIN; CFP10-DBD嵌合蛋白及其应用

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摘要

FIELD: biotechnologies.;SUBSTANCE: invention represents pCFP10-DBD recombinant plasmide consisting of an artificial bacterial operon of a chimeric protein including a promoter region of early promoter of T5 bacteriophage, a chimeric protein gene consisting of a sequence of protein antigene CFP10 from Mycobacterium tuberculosis, which is merged with a sequence of a dextrane-binding domain (DBD) of dextrane sucrase Leuconostoc citreum KM20, and a transcription terminator, a bacterial operon of beta-lactamase and a bacterial initiation section of replication of ColE1 type. Besides, strain Escherichia coli - a producer of CFP10-DBD chimeric protein is presented. The invention describes a method of immobilisation, concentration and cleaning of the obtained protein on cellulose. The invention describes an immunogenic composition containing CFP10-DBD recombinant protein, which is aimed at induction of immunity against tuberculous infection.;EFFECT: invention enlarges the range of devices used for treatment of tuberculosis.;4 cl, 6 dwg, 1 tbl, 4 ex

机译：领域：本发明代表由嵌合蛋白的人工细菌操纵子组成的pCFP10-DBD重组质粒，所述嵌合蛋白包括T5噬菌体的早期启动子的启动子区，所述嵌合蛋白基因由来自结核分枝杆菌的蛋白抗原CFP10的序列组成，该序列与葡聚糖蔗糖酶柠檬柠檬酸杆菌KM20的葡聚糖结合域（DBD）序列，转录终止子，β-内酰胺酶的细菌操纵子和ColE1型复制的细菌起始部分融合在一起。此外，还介绍了大肠杆菌-CFP10-DBD嵌合蛋白的生产者。本发明描述了将获得的蛋白质固定，浓缩和清洁在纤维素上的方法。本发明描述了包含CFP10-DBD重组蛋白的免疫原性组合物，其旨在诱导针对结核感染的免疫力。效果：本发明扩大了用于治疗结核的装置的范围; 4 cl，6 dwg，1 tbl，4前

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公开/公告号RU2546875C1

专利类型
公开/公告日2015-04-10

原文格式PDF
申请/专利权人 FEDERALNOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE TSENTRALNYJ NAUCHNO-ISSLEDOVATELSKIJ INSTITUT TUBERKULEZA ROSSIJSKOJ AKADEMII MEDITSINSKIKH NAUK;FEDERALNOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE NAUCHNO-ISSLEDOVATELSKIJ INSTITUT EHPIDEMIOLOGII I MIKROBIOLOGII IMENI POCHETNOGO AKADEMIKA N.F. GAMALEI MINISTERSTVA ZDRAVOOKHRANENIJA ROSSIJSKOJ FEDERATSII;
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申请/专利号RU20130151633
发明设计人 TKACHUK ARTEM PETROVICH;GINTSBURG ALEKSANDR LEONIDOVICH;LJASHCHUK ALEKSANDR MIKHAJLOVICH;APT ALEKSANDR SOLOMONOVICH;KARJAGINA-ZHULINA ANNA STANISLAVOVNA;POLETAEVA NINA NIKOLAEVNA;AKSENOVA EKATERINA IVANOVNA;SERGIENKO OLGA VASILEVNA;LUNIN VLADIMIR GLEBOVICH;GALUSHKINA ZOJA MIKHAJLOVNA;KONDRATEVA TATJANA KONSTANTINOVNA;EROKHIN VLADISLAV VSEVOLODOVICH;
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申请日2013-11-20
分类号C12N15/00;
国家 RU
入库时间 2022-08-21 14:56:27

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