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首页> 外文会议>Progress on post-genome technologies and modern natural products >CHARACTERIZATION OF RECOMBINANT SINGLE-STRAND BINDING PROTEIN FROM ESCHERICHIA COLI AND ITS APPLICATION IN PYROSEQUENCING
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CHARACTERIZATION OF RECOMBINANT SINGLE-STRAND BINDING PROTEIN FROM ESCHERICHIA COLI AND ITS APPLICATION IN PYROSEQUENCING

机译：大肠埃希菌重组单链结合蛋白的表征及其在热测序中的应用

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摘要

We expressed the recombinant single-strand binding protein (r-SSBP) from Escherichia coli with the molecular weight of 24- kDa by using the genetic engineering strategy. To characterize the r-SSBP, we studied the effects on melting temperature (Tm) of DNA. The results showed that the r-SSBP can bind to ssDNA, and lower the Tm of DNA, especially for single-base mismatched DNA. Therefore, the r-SSBP can significantly increase the Tm difference between single-base mismatched DNA and perfect matched DNA. These results are very beneficial for singlenucleotide polymorphism detection. Moreover, we applied the r-SSBP in high sensitive pyrosequencing system developed by our group. The results suggested that the r-SSBP can decrease unspecific signals and improve the performance of pyrosequencing. This research laid the foundation for the development of high sensitive pyrosequencing regents.
机译：我们通过使用基因工程策略从大肠杆菌中表达了分子量为24-kDa的重组单链结合蛋白（r-SSBP）。为了表征r-SSBP，我们研究了对DNA熔解温度（Tm）的影响。结果表明，r-SSBP可以与ssDNA结合，并降低DNA的Tm，特别是对于单碱基错配的DNA。因此，r-SSBP可以显着增加单碱基错配DNA与完全匹配DNA之间的Tm差异。这些结果对于单核苷酸多态性检测非常有益。此外，我们将r-SSBP应用于我们团队开发的高灵敏度焦磷酸测序系统。结果表明，r-SSBP可以减少非特异性信号并改善焦磷酸测序的性能。该研究为开发高灵敏度焦磷酸测序试剂奠定了基础。

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  • 来源
  • 会议地点 Chongqing(CN)
  • 作者

    Wang Jianping; Zou Bingjie; Chen Zhiyao; Ma Yinjiao; Xu Shu; Zhou Guohua;

  • 作者单位

    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China;

    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China;

    Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China;

    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China;

    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China;

    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物工程学（生物技术）;
  • 关键词

    Escherichia coli; single-strand binding protein; melting temperature; pyrosequencing;

    机译：大肠杆菌;单链结合蛋白熔点温度;焦磷酸测序;

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