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Genetic engineering techniques acidophilus and the resulting vector for transformation
Genetic engineering techniques acidophilus and the resulting vector for transformation
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机译：基因工程技术嗜酸菌及其转化载体
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摘要
1. A method for genetic engineering of acidophilic bacteria, which is characterized by the following steps: (1) preparing competent cells Acidithiobacillus ferrooxidans; (2) preparing a vector capable of self-replication when introduced into cells Acidithiobacillus ferrooxidans; (3) Transformation of Acidithiobacillus ferrooxidans cells previously entered into a temporary state of competence by elektroporatsii.2. Method for Genetic Engineering of acidophilic bacteria according to Claim. 1, which is characterized by the absence of changes in physiological characteristics bakteriy.3. Method for Genetic Engineering of acidophilic bacteria according to claim 1, characterized obtain competent cells, comprising the following steps: (a) seeding the growth phase standardization;. (B) filtering the cell; (C) a weakening or permeabilization of the bacterial cell wall for electrocompetent kletok.4. Method for Genetic Engineering of acidophilic bacteria n. 3, characterized by seeding the standardization phase of growth, which is a phase in which the crop reaches 50-80% oxidation srede.5 Fev. Method for Genetic Engineering of acidophilic bacteria n. 4, which is characterized by standardization seeding growth phase at 60-70% oxidation srede.6 Fev. Method for Genetic Engineering of acidophilic bacteria n. 3, which is characterized by a filtration step carried out using a 0.45 .mu.m membrane and followed by washing with physiological rastvorom.7. Method for Genetic Engineering of acidophilic bacteria n. 6, which is characterized by the physiological saline, which is a 0.9% w / v. NaCl in water and pH 5,8-7,5.8. Method for Genetic Engineering of acidophilic bacteria n. 3, which ha
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