首页> 外国专利> A METHOD FOR REGULATION OF CELLULAR BEHAVIORS BY APPLYING ELECTRIC STIMULUS BASED ON ENZYMATIC BIOFUEL CELL

A METHOD FOR REGULATION OF CELLULAR BEHAVIORS BY APPLYING ELECTRIC STIMULUS BASED ON ENZYMATIC BIOFUEL CELL

机译:一种基于酶生物燃料电池的电刺激调节细胞行为的方法

摘要

The present invention relates to a method for controlling cell behavior, comprising a step of using an enzymatic biofuel cell to apply electrical stimulation to cells; to a composition for inducing stem cell differentiation and a composition for promoting cell migration or proliferation; to a cell culturing container capable of culturing cells by comprising an enzymatic biofuel cell to apply electrical stimulation to cells; to a pharmaceutical composition containing an enzymatic biofuel cell for healing wounds, preventing or treating diseases caused by abnormal muscle control, bone diseases, and nerve disorders; and to muscle cells, bone cells, neuronal cells, and myocardial cells, which are differentiated by using the composition for inducing stem cell differentiation or the cell culturing container. According to the present invention, the method for controlling cell behavior by using an enzymatic biofuel cell to apply electrical stimulation to cells can increase applicability of a pharmaceutical composition in that biocompatibility can be increased by using an enzyme as an electrical stimulation source; a desired dosage form can be formulated; currents of various ranges can be generated by adjusting the concentration of the enzyme introduced in each electrode; and electrical stimulation can be generated without including both electrodes. Furthermore, when different levels of the electrical stimulation are applied at the level at which apoptosis is not induced, stem cells can be differentiated into different tissues, thereby allowing the stem cells to be variously formulated and useful for tissue regeneration.
机译:控制细胞行为的方法技术领域本发明涉及一种控制细胞行为的方法,该方法包括以下步骤:使用酶促生物燃料电池对细胞施加电刺激。诱导干细胞分化的组合物和促进细胞迁移或增殖的组合物;能够通过包含酶生物燃料电池以对细胞施加电刺激来培养细胞的细胞培养容器;包含酶生物燃料电池的药物组合物,用于愈合伤口,预防或治疗由异常肌肉控制,骨骼疾病和神经疾病引起的疾病;通过使用诱导干细胞分化的组合物或细胞培养容器来分化的肌肉细胞,骨细胞,神经元细胞和心肌细胞。根据本发明,通过使用酶促生物燃料电池对细胞进行电刺激来控制细胞行为的方法可以提高药物组合物的适用性,因为可以通过使用酶作为电刺激源来提高生物相容性。可以配制所需的剂型;通过调节引入每个电极的酶的浓度,可以产生各种范围的电流。无需两个电极即可产生电刺激。此外,当在不诱导凋亡的水平上施加不同水平的电刺激时,干细胞可以分化成不同的组织,从而允许干细胞被不同地配制并且可用于组织再生。

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