首页> 外国专利> SAMPLE NUCLEIC ACID FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, PCR PRIMER FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, AND METHOD FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES WHICH CAN BE USED IN ION EXCHANGE CHROMATOGRAPHIC ANALYSIS

SAMPLE NUCLEIC ACID FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, PCR PRIMER FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, AND METHOD FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES WHICH CAN BE USED IN ION EXCHANGE CHROMATOGRAPHIC ANALYSIS

机译：用于单核苷酸多态性检测目的的样品核酸，用于制备单核苷酸多态性检测样品的PCR引物，以及用于制备单核苷酸多态性分析目的肽段的方法

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摘要

An object of the present invention is to provide a sample nucleic acid for single nucleotide polymorphism detection which is for use in a simple method for quickly detecting a single nucleotide polymorphism, PCR primers for preparation of a sample for single nucleotide polymorphism detection, and a sample for single nucleotide polymorphism detection which is for use in ion exchange chromatography analysis. The present invention provides a sample nucleic acid for single nucleotide polymorphism detection having the following features: (a) a full chain length of 200 bp or less; (b) a sequence (a tag sequence) incompletely complementary to a template DNA at the 5' or 3' end; and (c) a 10 bp or less difference in chain length from a sample nucleic acid to be compared with to determine the presence of a single nucleotide polymorphism.

机译：本发明的目的是提供用于单核苷酸多态性检测的样品核酸，其用于快速检测单核苷酸多态性的简单方法，用于制备用于单核苷酸多态性检测的样品的PCR引物和样品。用于单核苷酸多态性检测，用于离子交换色谱分析。本发明提供了具有以下特征的用于单核苷酸多态性检测的样品核酸：（a）200 bp或更短的全长链;（b）在5'或3'端与模板DNA不完全互补的序列（标签序列）;和（c）与要比较的样品核酸的链长相差10 bp或更小，以确定单核苷酸多态性的存在。

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公开/公告号EP2692863A4

专利类型
公开/公告日2015-02-25

原文格式PDF
申请/专利权人 SEKISUI MEDICAL CO. LTD.;
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申请/专利号EP20120765405
发明设计人 YOTANI TAKUYA;KIYOTOH EIJI;USHIZAWA KOJI;
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申请日2012-03-30
分类号G01N30/88;C12N15;C12Q1/68;G01N30/26;
国家 EP
入库时间 2022-08-21 15:06:20

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