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homogeneous assay and assay kit for the determination of aflatoxins in agricultural products
homogeneous assay and assay kit for the determination of aflatoxins in agricultural products
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机译：均相测定法和测定试剂盒，用于测定农产品中的黄曲霉毒素
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摘要
"homogeneous assay and assay kit for the determination of aflatoxins in agricultural products". A homogeneous assay to determine aflatoxin content in agricultural products uses the fluorescence polarization technique. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracker and an aflatoxin-specific monoclonal antibody. The tracker is capable of binding to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracker is prepared by conjugating an aflatoxin oxime to a suitable fluorophor. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture can be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
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