首页>
外国专利>
Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes
Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes
展开▼
机译：利用pnipaam包裹酶的生物传感器titanium氧化钛传感器膜的检测方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1×PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.
展开▼
机译：通过使用PNIPAAm包裹酶来检测由euro氧化钛作为生物传感器组成部分的传感器膜的方法，该方法包括将1.0 g NIPAAm粉末添加到20 ml水中，在60°C加热以形成NIPAAm溶液，然后将其冷却NIPAAm解决方案;向NIPAAm溶液中加入200μl98.7 wt％的APS和50μl99 wt％的TEMED，将其均匀混合，并使混合物反应30小时，以制备透明的凝胶PNIPAAm;在100μl的1×PBS缓冲溶液中加入5mg酶，均匀混合，在缓冲溶液中加入100μl的PNIPAAm，均匀混合。将生物传感器放置在加热器上以在37℃恒温加热，该生物传感器是具有由EuTi x O y 形成的传感器膜的EIS传感器;并进行测量。
展开▼
