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Rice gene capable of imparting wide-spectrum disease resistance

机译：具有广谱抗病性的水稻基因

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摘要

A three-step screening of selection for resistance against pathogenic bacteria infection, selection for resistance against pathogenic fungi infection, and selection for sensitivity to salicylic acid was carried out on Arabidopsis thaliana lines highly expressing rice full-length cDNA (rice-FOX Arabidopsis lines) which were prepared by using a FOX hunting system. As a result, one Arabidopsis thaliana line (line K15424) selected in all of the three types of screening was successfully selected. Line K15424 carries a rice full-length cDNA. Rice overexpressing AK070024 was produced, and resistance against rice bacterial leaf blight was assayed using the T1 generation. As a result, it was confirmed that AK070024-overexpressing rice is resistant against bacterial leaf blight and blast.

机译：在高表达水稻全长cDNA的 Arabidopsis thaliana 品系上进行了三步筛选，筛选抗病原菌感染，抗病原真菌感染和对水杨酸敏感性的筛选。（FOX 拟南芥系）通过使用FOX狩猎系统制备。结果，成功地选择了在所有三种类型的筛选中选择的一条拟南芥品系（品系K15424）。品系K15424携带水稻全长cDNA。产生了水稻过表达的AK070024，并使用T1代测定了对水稻细菌性叶枯病的抗性。结果，证实了AK070024-过表达的水稻对细菌性叶枯病和稻瘟病具有抗性。 展开▼

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公开/公告号US9127290B2

专利类型

公开/公告日2015-09-08

原文格式PDF

申请/专利权人 MASAKI MORI;NAGAO HAYASHI;SHOJI SUGANO;HIROSHI TAKATSUJI;HIROHIKO HIROCHIKA;KENJI ODA;MINAMI MATSUI;
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申请/专利号US200913061396

发明设计人 MASAKI MORI;NAGAO HAYASHI;SHOJI SUGANO;HIROSHI TAKATSUJI;HIROHIKO HIROCHIKA;KENJI ODA;MINAMI MATSUI;
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申请日2009-03-04

分类号A01H5;C12Q1/02;C12N15/82;C12N5/10;C12N9/12;G01N33/50;

国家 US

入库时间 2022-08-21 15:18:47

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