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METHODS FOR DETECTING ASPERGILLUS OCHRACEUS AND ASPERGILLUS WESTERDIJKIAE

机译:蛇骨和西部无花果的检测方法

摘要

PROBLEM TO BE SOLVED: To provide rapid and accurate methods for detecting Aspergillus ochraceus and Aspergillus westerdijkiae at the respective species levels.SOLUTION: There are provided two kinds of oligonucleotides with deletion, substitution, insertion or addition of one or several bases in specified base sequences and usable for detection of Aspergillus ochraceus, and two kinds of oligonucleotides with deletion, substitution, insertion or addition of one or several bases in specified base sequences and usable for detection of Aspergillus westerdijkiae. Also provided are a method for detecting Aspergillus ochraceus and Aspergillus westerdijkiae at the respective species levels comprising amplifying a part of a nucleic acid having a base sequence of at least one -tubulin gene, and confirming the presence or absence of the amplified products.
机译:解决的问题:提供快速,准确的方法来检测各个物种水平上的och曲霉和西曲霉解决方案:提供了两种寡核苷酸,它们在指定碱基序列中具有缺失,取代,插入或添加一个或多个碱基可用于检测曲霉,以及在指定碱基序列中缺失,取代,插入或添加一个或多个碱基的两种寡核苷酸,可用于检测西曲霉。还提供了一种用于在各个物种水平上检测曲霉和西曲霉的方法,该方法包括扩增具有至少一个微管蛋白基因的碱基序列的核酸的一部分,并确认扩增产物的存在或不存在。

著录项

  • 公开/公告号JP2015195774A

    专利类型

  • 公开/公告日2015-11-09

    原文格式PDF

  • 申请/专利权人 KAO CORP;

    申请/专利号JP20140076418

  • 申请日2014-04-02

  • 分类号C12N15/09;C12Q1/68;

  • 国家 JP

  • 入库时间 2022-08-21 15:33:52

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