首页>
外国专利>
ISOTOPE LABELED WITH THE NEW MASS VALUES N-LINKED GLYCANS, A METHOD FOR MANUFACTURING THE SAME, AND THE RELATIVE QUANTIFICATION OF ISOTOPE LABELED N-LINKED GLYCAN BY MASS SPECTROMETRY METHODS
ISOTOPE LABELED WITH THE NEW MASS VALUES N-LINKED GLYCANS, A METHOD FOR MANUFACTURING THE SAME, AND THE RELATIVE QUANTIFICATION OF ISOTOPE LABELED N-LINKED GLYCAN BY MASS SPECTROMETRY METHODS
The present invention provides a method for comparative quantitative mass spectrometric analysis of a N-linked glycan hydrolyzed and transglycosylated by adding an enzyme, endo--N-acetylglucosaminidases (Endo-M), to a glycoprotein. The N-linked glycan having a new mass value of 1390.18 m/z can be used as an internal standard for comparative quantitative analysis of glycans. Therefore, the present invention is applicable for comparative quantification and structural analysis of an isotope-labeled N-linked glycan by the method of the present invention.
展开▼
机译：本发明提供了一种比较定量质谱分析的方法，该方法通过向糖蛋白中加入内切-N-乙酰氨基葡萄糖苷酶（Endo-M）来水解和转糖基化的N-连接的聚糖。新质量值为1390.18 m / z的N-连接聚糖可用作内标，用于比较定量分析聚糖。因此，本发明适用于通过本发明的方法对同位素标记的N-连接的聚糖进行比较定量和结构分析。
展开▼
