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Method for preparing an insoluble product of Mucor racemosus lipase

机译:消旋毛霉脂肪酶不溶产物的制备方法

摘要

process to obtain insoluble of mucor lipase racemosus katalizujacego hydrolize oligolipidu00f3w is breeding selektanta mold mucor racemosus a33 / ppdgp,the spores are in constant quality of the terrain zawierajacym agar, brzeczke piwowarska conventions and polyethylene glycol distearate.inoculum cultures selektanta is in the quality of the terrain cieklym zawierajacym oil oil or corn oil, namok, water wodociagowa and polyethylene glycol distearate.production farms selektanta leads to quality of the terrain cieklym zawierajacym oil oil or corn oil, namok and water wodociagowa,the inoculated inoculum when mixing zawartosci fermenter with mieszadla,which is fixed to rozlacznie porous nosnik bred selektanta as prostopadlosciennych platu00f3w polyurethane foam.and obtained by breeding plata foam from przerosnieta biomass selektanta mucor racemosus a33 / ppdgp separates from the liquid pohodowlanej, washed to distilled water.odwadnia schlodzonym acetone, petroleum ether is extracted and dried in room temperature or is washed with distilled water, zamraza, liofilizuje, washed with acetonepetroleum ether and dried at room temperature.selektant mold mucor racemosus a33 / ppdgp is replaced with a mold mucor racemosus a33, which is subjected to activation at constant quality of the terrain about every game: polyethylene glycol distearate, agar,brzeczka piwowarska.then wyrosle colonies on quality of the terrain being constant selection by breeding wstrzasanej on quality of the terrain cieklym about every game: polyethylene glycol distearate, olive oil or oilnamok corn, water wodociagowa with procedure activation and selection of mold mucor racemosus repeats up 3 times.the selektant store on the quality of the terrain constant for every game: polyethylene glycol distearate, agar, brzeczka piwowarska, temperature 4u00b0c.
机译:获得不溶性粘胶脂肪酶消旋体的过程,katalizujacego水解了寡脂,正在繁殖粘粒消旋体a33 ppdgp,孢子处于稳定的地形zawierajacym琼脂,brzeczke piwowarantasates和聚乙烯乙二醇。地形citklym zawierajacym油或玉米油,namok,水wodociagowa和聚乙二醇二硬脂酸酯的质量。生产农场selektanta导致地形cieklym zawierajacym油或玉米油,namok和水wodociagowa混合zawartosmenter时接种的接种物用mieszadla固定在rozlacznie多孔nosnik繁殖的selektanta上,作为prostopadlosciennych plat聚氨酯泡沫,并通过从przerosnieta生物质selektanta mucor racemosus a33 / ppdgp育成的plata泡沫获得,与液体水生多酚乙草胺(蒸馏水)分离得到。提取石油醚并在室温下干燥或用蒸馏水,Zamraza,liofilizuje洗涤,用丙酮石油醚洗涤并在室温下干燥。用霉菌毛霉消霉菌a33代替精制的霉菌毛霉消灭草a33 / ppdgp,该霉菌以恒定的质量进行活化每场比赛的地形:聚乙二醇二硬脂酸酯,琼脂,brzeczka piwowarska。然后,通过对每场比赛的地形质量进行wstrzasanej繁殖,不断选择关于地形质量的wyrosle菌落:聚乙二醇二硬脂酸酯,橄榄油或Oilnamok玉米,水wodociagowa与程序激活和模具粘液消旋的选择重复3次。selektant存储在每场比赛的地形常数恒定:聚乙二醇二硬脂酸酯,琼脂,brzeczka piwowarska,温度4 u00b0c。

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