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Methods, reagents and instrumentation for preparing impregnated tissue samples suitable for histopathological and molecular studies
Methods, reagents and instrumentation for preparing impregnated tissue samples suitable for histopathological and molecular studies
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机译:用于制备适合组织病理学和分子研究的浸渍组织样品的方法,试剂和仪器
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摘要
A process and kit for the production of paraffin sections of biological tissue, especially for molecular pathology studies, is described. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. The method is uncomplicated, requires little hands-on time, is reproducible and is standardized thereby limiting cross-center pre-analytical variance. A bio-indicator system for measuring the degree of crosslinking is also disclosed.
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