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COMPOSITIONS FOR THE IN VITRO DERIVATION AND CULTURE OF EMBRYONIC STEM (ES) CELL LINES WITH GERMLINE TRANSMISSION CAPABILITY AND FOR THE CULTURE OF ADULT STEM CELLS
COMPOSITIONS FOR THE IN VITRO DERIVATION AND CULTURE OF EMBRYONIC STEM (ES) CELL LINES WITH GERMLINE TRANSMISSION CAPABILITY AND FOR THE CULTURE OF ADULT STEM CELLS
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机译：具有生殖细胞传代能力的胚胎干细胞系的体外衍生和培养以及成人干细胞培养的组合物
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摘要
The present invention is directed to a method of deriving pluripotent embryonic stem cells from mouse blastocysts or from primordial germ cells from a post-implantation mouse embryo, or of maintaining or growing pluripotent embryonic stem cells from a mouse, or of expanding human hematopoietic stem cells or human hematopoietic precursor cells. The methods include the step of cultivating the stem cells or precursor cells for at least one passage in a culture medium preconditioned by the rabbit fibroblast cell line Rab9 (ATCC catalogue CRL1414) and containing less than 0.1 ng/ml Leukemia Inhibitory Factor (LIF).
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