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Synthetic surfaces for culturing cells in a chemically defined medium

机译:用于在化学成分确定的培养基中培养细胞的合成表面

摘要

Synthetic surface capable of supporting the cultivation of eukaryotic cells, including stem cells and undifferentiated human embryonic stem cells in chemically defined medium comprises a swellable (meth) acrylate layer and the polypeptide bound to the swellable (meth) acrylate . The swellable (meth) acrylate layer, the carboxyl group-containing (meth) acrylate monomer, a crosslinkable (two or higher-functional) (meth) acrylate monomers, and carboxyl group-containing (meth) acrylate monomer and the crosslinking ( it may be formed by monomer polymerization in a composition comprising a hydrophilic monomer capable of polymerizing with meth) acrylate monomers. Swellable (meth) acrylate layer has an equilibrium moisture content in water of between about 5% and about 70%. Binding peptide will comprise an RGD amino acid sequence.
机译:能够支持在化学成分确定的培养基中培养真核细胞(包括干细胞和未分化的人类胚胎干细胞)的合成表面包含可溶胀的(甲基)丙烯酸酯层和与可溶胀的(甲基)丙烯酸酯结合的多肽。可溶胀的(甲基)丙烯酸酯层,含羧基的(甲基)丙烯酸酯单体,可交联的(两个或更高官能度的)(甲基)丙烯酸酯单体和含羧基的(甲基)丙烯酸酯单体和交联(通过在包含能够与甲基)丙烯酸酯单体聚合的亲水性单体的组合物中的单体聚合反应来形成丙烯酸酯。可溶的(甲基)丙烯酸酯层在水中的平衡水分含量为约5%至约70%。结合肽将包含RGD氨基酸序列。

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