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METHOD FOR CRYSTALLIZING ACINETOBACTER BAUMANNII PEPTIDOGLYCAN-CONJUGATED LIPOPROTEIN

机译:结晶鲍曼不动杆菌肽糖聚糖缀合脂蛋白的方法

摘要

PURPOSE: A method for crystallizing Acinetobacter baumannii peptidoglycan-conjugated lipoprotein is provided to enable easy X-ray analysis, and to obtain a single crystal of protein with excellent crystallinity.;CONSTITUTION: A method for crystallizing Acinetobacter baumannii peptidoglycan-conjugated lipoprotein comprises the step of crystallizing by a vapour diffusion method using a crystallization solution containing potassium monohydrogen phosphate (K2HPO4) and sodium dihydrogen phosphate (NaH2PO4) as precipitants and phosphate citrate as a buffer solution. The lipoprotein is prepared by fusing 4-10 histidine tags to a protein for easy purification at an amino terminal. The precipitant is 0.01-10 M of potassium monohydrogen phosphate and 0.01-10 M of sodium dihydrogenphosphate.;COPYRIGHT KIPO 2013
机译:目的:提供一种使鲍曼不动杆菌肽聚糖结合的脂蛋白结晶的方法,以便于X射线分析,并获得具有优良结晶度的蛋白质单晶。使用包含磷酸一氢钾(K2HPO4)和磷酸二氢钠(NaH2PO4)作为沉淀剂和柠檬酸磷酸酯作为缓冲溶液的结晶溶液通过蒸汽扩散法进行结晶的方法。脂蛋白的制备方法是将4-10个组氨酸标签融合到蛋白质上,以便在氨基末端轻松纯化。沉淀剂是0.01-10 M的磷酸一氢钾和0.01-10 M的磷酸二氢钠。; COPYRIGHT KIPO 2013

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