首页> 外国专利> Culture method for amplifying large numbers of hair follicle stem cells in vitro

Culture method for amplifying large numbers of hair follicle stem cells in vitro

机译:体外扩增大量毛囊干细胞的培养方法

摘要

The present invention is a method for amplifying a large numbers of hair follicle stem cells in vitro by using microspheres as carriers for cell culture and a revolving bottle as a fermentation tank for cell proliferation. The method includes digesting hair follicles to obtain hair follicle stem cells, seeding the cells on microcarriers, transferring the microcarriers to a revolving bottle, feeding liquid culture media into the revolving bottle, placing the revolving bottle into a cell incubator, growing the cells on the microcarrier and harvesting the hair follicle stem cells.
机译:本发明是一种通过使用微球作为细胞培养的载体并且使用旋转瓶作为细胞增殖的发酵罐体外扩增大量毛囊干细胞的方法。该方法包括消化毛囊以获得毛囊干细胞,将细胞接种在微载体上,将微载体转移到旋转瓶中,将液体培养基注入旋转瓶中,将旋转瓶放入细胞培养箱中,在微载体并收获毛囊干细胞。

著录项

  • 公开/公告号US8524492B2

    专利类型

  • 公开/公告日2013-09-03

    原文格式PDF

  • 申请/专利权人 JINYU LIU;YULIN LI;

    申请/专利号US201113517068

  • 发明设计人 JINYU LIU;YULIN LI;

    申请日2011-07-20

  • 分类号C12N5/00;

  • 国家 US

  • 入库时间 2022-08-21 16:45:14

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号