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FOOD-POISONING BACTERIA DETECTION CARRIER, AND METHOD FOR DETECTING FOOD-POISONING BACTERIA
FOOD-POISONING BACTERIA DETECTION CARRIER, AND METHOD FOR DETECTING FOOD-POISONING BACTERIA
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机译:食品中毒细菌检测载体和食品中毒细菌检测方法
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摘要
A plurality of probes are immobilized on a carrier for detecting food poisoning bacteria, the plurality of probes being selected, either alone or in combination, respectively from two or more groups among a first probe group for detecting Escherichia coli including probes that respectively have base sequences of SEQ ID NO: 1 to 6, and probes that respectively have base sequences complementary to the base sequences of SEQ ID NO: 1 to 6 (hereinafter referred to as “complementary probes”), a second probe group for detecting Listeria including probes that respectively have base sequences of SEQ ID NO: 7 to 13, and complementary probes, a third probe group for detecting Campylobacter including probes that respectively have base sequences of SEQ ID NO: 14 to 19, and complementary probes, a fourth probe group for detecting Vibrio parahaemolyticus including probes that respectively have base sequences of SEQ ID NO: 20 to 24, and complementary probes, a fifth probe group for detecting Staphylococcus aureus including probes that respectively have base sequences of SEQ ID NO: 25 to 29, and complementary probes, a sixth probe group for detecting Salmonella including probes that respectively have base sequences of SEQ ID NO: 30 to 40, and complementary probes, and a seventh probe group for detecting Bacillus cereus including probes that respectively have base sequences of SEQ ID NO: 41 to 49, and complementary probes. Two or more types of food poisoning bacteria among Escherichia coli, Listeria, Campylobacter, Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella, and Bacillus cereus can be specifically and simultaneously detected using the carrier.
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机译:将多个探针固定在用于检测食物中毒细菌的载体上,所述多个探针分别单独或组合地选自用于检测大肠杆菌的第一探针组中的两个或更多个组。包括分别具有SEQ ID NO:1至6的碱基序列的探针和分别具有与SEQ ID NO:1至6的碱基序列互补的碱基序列的探针(以下称为“互补探针”),第二探针用于检测李斯特菌 I>的组,包括分别具有SEQ ID NO:7至13的碱基序列的探针,以及互补探针,用于检测弯曲杆菌 I>的第三种探针组,包括分别具有SEQ ID NO:14至19的碱基序列,以及互补探针,用于检测副溶血弧菌的第四探针组,包括分别具有SEQ ID NO:20至24碱基序列的探针,以及互补探针,第五探针g用于检测金黄色葡萄球菌 I>的程序,包括分别具有SEQ ID NO:25至29的碱基序列的探针,以及互补探针,用于检测沙门氏菌 I>的第六探针组,包括分别具有具有SEQ ID NO:30至40的碱基序列和互补探针,以及用于检测蜡状芽孢杆菌的第七探针组,包括分别具有SEQ ID NO:41至49的碱基序列的探针和互补探针。使用载体可以特异性地同时检测出大肠杆菌,李斯特菌,弯曲杆菌,副溶血性弧菌,金黄色葡萄球菌,沙门氏菌 I>和蜡状芽孢杆菌 I>中的两种或两种以上食物中毒细菌。 。
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