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METHOD AND KIT FOR IDENTIFYING A TRANSLATION INITIATION SITE ON AN MRNA
METHOD AND KIT FOR IDENTIFYING A TRANSLATION INITIATION SITE ON AN MRNA
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机译：识别mRNA翻译起始位点的方法和试剂盒
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摘要
The present invention relates to a method and kit for identifying a translation initiation site on an mRNA. The method involves contacting a first mRNA with a first translation inhibitor to preferentially stabilize one or more initiation ribosomes at translation initiation sites on the first mRNA. A second mRNA is contacted with a second translation inhibitor different from the first translation inhibitor to stabilize one or more initiation ribosomes and one or more elongation ribosomes on the second mRNA. The location of ribosomes stabilized on the first mRNA is compared to the location of ribosomes stabilized on the second mRNA.
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