首页> 外国专利> New non-human transgenic animal having incorporated into its genome a transgene comprising reporter gene linked to regulatory sequences, useful to investigate effect of environmental stimulus of eukaryotic initiation factor 2-alpha

New non-human transgenic animal having incorporated into its genome a transgene comprising reporter gene linked to regulatory sequences, useful to investigate effect of environmental stimulus of eukaryotic initiation factor 2-alpha

机译：一种新的非人类转基因动物，其基因组中整合了包含与调控序列连接的报告基因的转基因，可用于研究环境刺激对真核起始因子2-alpha的影响

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摘要

Non-human transgenic animal having incorporated into its genome a transgene comprising a reporter gene linked to regulatory sequences of cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer-binding protein (EBP)-activating transcription factor (ATF) responsive element (CARE) and a ubiquitous promoter, is new. An independent claim is included for screening substances capable of acting on the signaling pathway eukaryotic initiation factor (eIF)2alpha /ATF4, comprising inducing an environmental stimulus in the transgenic animal, measuring the level of expression of the reporter gene under the control of the sequence CARE included in the transgene, administering a substance to the animal, measuring the level of expression of the reporter gene under the control of the sequence CARE included in the transgene after administration of the substance, where the substance is a substance of interest if it is on the transgenic animal by restoring in the animal an expression similar to the expression of the reporter gene in the same transgenic animal not subjected to the stimulus, or by increasing the expression of the reporter gene in the transgenic animal subjected to the stimulus.

机译：非人类转基因动物已在其基因组中整合了一个转基因，该转基因包含一个报告基因，该报告基因与胞苷-胞苷-腺苷-腺苷-胸苷（CCAAT）/增强子结合蛋白（EBP）激活转录因子（ATF）响应元件的调控序列连接（CARE）和无处不在的启动子，是新的。包括独立的权利要求用于筛选能够作用于真核起始因子（eIF）2alpha / ATF4信号转导途径的物质，包括在转基因动物中诱导环境刺激，在序列控制下测量报告基因的表达水平转基因中包含的CARE，向动物给药，在给药后在转基因中包含的CARE序列的控制下，测量报告基因的表达水平，如果该物质是目标物质，则为通过在动物中恢复与未受刺激的同一转基因动物中的报道基因表达相似的表达，或通过增加受刺激的转基因动物中的报告基因表达，来在转基因动物中恢复表达。

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公开/公告号FR2972605A1

专利类型
公开/公告日2012-09-21

原文格式PDF
申请/专利权人 INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE (INRA);CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);
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申请/专利号FR20110000775
发明设计人 BRUHAT ALAIN;FAFOURNOUX PIERRE;
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申请日2011-03-15
分类号A01K67/027;C12N15/85;C12Q1/02;C12Q1/66;
国家 FR
入库时间 2022-08-21 17:04:00

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