首页> 外国专利> NUCLEOTIDE SEQUENCE, CODING IMMUNOGENIC POLYPEPTIDE LcrV(G113), CAUSING PROTECTIVE IMMUNE RESPONSE AGAINST Yersinia pestis; RECOMBINANT PLASMID DNA pETV-I-3455, CODING IMMUNOGENIC POLYPEPTIDE LcrV(G113); RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pETV-I-3455 - PRODUCER OF IMMUNOGENIC POLYPEPTIDE LcrV(G113); POLYPEPTIDE LcrV(G113) AND METHOD OF ITS OBTAINING

NUCLEOTIDE SEQUENCE, CODING IMMUNOGENIC POLYPEPTIDE LcrV(G113), CAUSING PROTECTIVE IMMUNE RESPONSE AGAINST Yersinia pestis; RECOMBINANT PLASMID DNA pETV-I-3455, CODING IMMUNOGENIC POLYPEPTIDE LcrV(G113); RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pETV-I-3455 - PRODUCER OF IMMUNOGENIC POLYPEPTIDE LcrV(G113); POLYPEPTIDE LcrV(G113) AND METHOD OF ITS OBTAINING

机译：核苷酸序列，编码免疫原性多肽LcrV（G113），引起鼠疫耶尔森氏菌的保护性免疫反应;重组质粒DNA pETV-I-3455，编码免疫原性多肽LcrV（G113）;重组菌株大肠杆菌BL21（DE3）/ pETV-I-3455-免疫原多肽LcrV（G113）的生产商;多肽LcrV（G113）及其获得方法

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FIELD: medicine.;SUBSTANCE: invention describes nucleotide sequence (dwg.2), coding immunogenic polypeptide LcrV(G113), serving the base for construction of recombinant plasmid DNA pETV-I-3455, with the size 6538 bp, which codes immunogenic polypeptide LcrV(G113). Plasmid consists of plasmid pBR322 replicon, β-lactamase gene, determining resistance to ampicillin, T7-promoter, 1ac-operator, f1-replicon and DNA fragment, flanked by the sites for restrictases Ndel and Hindlll, coding synthesis of protein LcrV(G113), which starts from initiating codon ATG. Described is recombinant strain of bacteria E. coli BL21 (DE3)/pETV-I-3455 - producer of immunogenic polypeptide LcrV(G113) with amino acid sequence, represented on dwg.3, where tryptophan in position 113 (W113) is substituted with glycin. Described is method of obtaining said polypeptide by cultivation of strain E. coli BL21(DE3)/pETV-I-3455. Cells are destroyed in buffer solution by ultrasound and polypeptide is isolated successively by gel-permeation chromatography with application of carrier TSK HW-40, anion-exchanging and hydrophobic chromatography.;EFFECT: invention makes it possible to obtain product with high immunogenic and protective activity.;5 cl, 10 dwg, 2 tbl, 5 ex

机译：技术领域：本发明描述了编码免疫原性多肽LcrV（G113）的核苷酸序列（dwg.2），为构建编码免疫原性多肽的大小为6538 bp的重组质粒DNA pETV-I-3455提供了基础LcrV（G113）。质粒由质粒pBR322复制子，β-内酰胺酶基因（确定对氨苄青霉素，T7启动子，1ac-操纵子，f1-复制子和DNA片段的抗性）组成，两侧是限制性酶Ndel和Hindlll的位点，编码蛋白LcrV（G113）的合成，从启动密码子ATG开始。描述了细菌大肠杆菌BL21（DE3）/ pETV-I-3455的重组菌株-带有氨基酸序列的免疫原性多肽LcrV（G113）的生产者，在dwg.3上代表，其中113位（W113）的色氨酸被替换为甘氨酸。描述了通过培养大肠杆菌BL21（DE3）/ pETV-I-3455菌株获得所述多肽的方法。通过超声破坏缓冲溶液中的细胞，并通过使用载体TSK HW-40的凝胶渗透色谱，阴离子交换和疏水色谱相继分离多肽;效果：本发明使获得具有高免疫原性和保护活性的产品成为可能。; 5 cl，10 dwg，2 tbl，5 ex

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公开/公告号RU2439155C1

专利类型
公开/公告日2012-01-10

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申请/专利权人 FEDERALNOE GOSUDARSTVENNOE UCHREZHDENIE NAUKI GOSUDARSTVENNYJ NAUCHNYJ TSENTR PRIKLADNOJ MIKROBIOLOGII I BIOTEKHNOLOGII (FGUN GNTS PMB);
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申请/专利号RU20100120197
发明设计人 KOPYLOV PAVEL KHRISTOFOROVICH;BAKHTEEVA IRINA VIKTOROVNA;ANISIMOV ANDREJ PAVLOVICH;DENTOVSKAJA SVETLANA VLADIMIROVNA;IVANOV SERGEJ ANDREEVICH;KISELEVA NATALJA VLADIMIROVNA;LEVCHUK VLADIMIR PAVLOVICH;PANFERTSEV EVGENIJ ALEKSANDROVICH;PLATONOV MIKHAIL EVGENEVICH;SVETOCH TATJANA EHDUARDOVNA;TITAREVA GALINA MIKHAJLOVNA;
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申请日2010-06-02
分类号C12N15/10;C07H21;C12N15/70;C12N1/21;C12P21;C12R1/19;
国家 RU
入库时间 2022-08-21 17:06:20

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