首页> 外国专利> Recombinant plasmid DNA pMSIN4, encoding a hybrid polypeptide comprising human insulin precursor, E. coli strain BL21 (DE3) / pMSIN4 - producer of recombinant human insulin, the method for the recombinant human insulin production

Recombinant plasmid DNA pMSIN4, encoding a hybrid polypeptide comprising human insulin precursor, E. coli strain BL21 (DE3) / pMSIN4 - producer of recombinant human insulin, the method for the recombinant human insulin production

机译:重组质粒DNA pMSIN4,编码包含人胰岛素前体的杂合多肽,大肠杆菌菌株BL21(DE3)/ pMSIN4-重组人胰岛素的生产者,生产重组人胰岛素的方法

摘要

The invention refers to biotechnology, particularly genetic engineering, and can be used in the production of recombinant human insulin.;The technical result, at which the invention is aimed, is to increase the quality and quantity of yield of the desired product - recombinant human insulin, as well as to simplify the industrial production of recombinant human insulin.;The result is achieved by constructing a recombinant plasmid pMSIN4, encoding a hybrid polypeptide - a precursor of human insulin, a strain VL21 (DE3) / pMSIN4 - producer of recombinant human insulin, as well as by optimizing the way of the industrial production of recombinant human insulin.;The inventions therefore allows to simplify the process of the industrial production of the high purity recombinant human insulin, as well as to increase the yield of the desired product.
机译:本发明涉及生物技术,特别是基因工程,并且可用于生产重组人胰岛素。本发明的目的是提高所需产物-重组人的质量和产量。 ;以及简化重组人胰岛素的工业生产。;结果是通过构建重组质粒pMSIN4来实现的,该质粒编码杂合多肽-人胰岛素的前体,菌株VL21(DE3)/ pMSIN4-重组体的生产者人胰岛素,以及通过优化重组人胰岛素的工业化生产方式。因此,本发明允许简化高纯度重组人胰岛素的工业化生产过程,并提高所需产量。产品。

著录项

  • 公开/公告号EP2502633A1

    专利类型

  • 公开/公告日2012-09-26

    原文格式PDF

  • 申请/专利权人 WINSORT MANAGEMENT INC.;

    申请/专利号EP20120156526

  • 发明设计人

    申请日2012-02-22

  • 分类号A61K38/28;C07K14/62;C12P21/02;

  • 国家 EP

  • 入库时间 2022-08-21 17:11:45

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