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COMPOSITIONS AND METHODS FOR GENE EXPRESSION AND CHROMATIN PROFILING OF INDIVIDUAL CELL TYPES WITHIN A TISSUE
COMPOSITIONS AND METHODS FOR GENE EXPRESSION AND CHROMATIN PROFILING OF INDIVIDUAL CELL TYPES WITHIN A TISSUE
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机译：组织内单个细胞类型的基因表达和染色质形成的组合物和方法
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摘要
The present invention provides compositions, methods, and kits for generating and isolating tagged nuclei of specific cell types with a high yield and purity. The compositions, methods, and kits provided herein comprise expressing in a cell a nuclear envelope tagging fusion polypeptide comprising a nuclear envelope targeting domain and an affinity reagent binding region, under the control of a cell type- specific promoter. Some embodiments also comprise expressing in a cell a biotin ligase, wherein the affinity reagent binding region comprises a biotin ligase accepting site, and wherein at least one of the nuclear envelope tagging fusion polypeptide and the biotin ligase is expressed under the control of a cell type-specific promoter.
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